Comparison of the Biochemical and Immunological Properties of Nerve Growth Factors from Various Animals
نویسندگان
چکیده
Nerve growth factors (NGF's) were purified from mouse submaxillary gland, guinea pig prostate gland, bovine seminal plasma, and venom of Naja naja atra, and their biochemical and immunological properties were compared. The NGF's from the four sources stimulated the maximal response of nerve fiber outgrowth from chick dorsal root ganglia at 10 ng/ml. Two-site enzyme immunoassay (ETA) systems for these NGF's showed that mouse NGF, guinea pig NGF, and bovine NGF were similar in immunological properties and that the crossreactivity was less than 1 % between snake NGF and mammalian NGF's. However, pretreatment with anti-snake NGF antibody inhibited the biological activity of mammalian NGF's even when their immunological activity was not completely inhibited. These results suggest that snake NGF and mammalian NGF's are similar in their biological activity but not in their immunological activity and that anti-snake NGF antibody recognizes the parts of mammalian NGF's essential for the biological activity.
منابع مشابه
The comparison of normal venous blood with
Introduction: Cupping therapy (hejamat) is one of the therapeutic methods used in traditional medicine that it is still common in many of the contemporary societies. In spite of the progress of science, we have less information on the traditional and alternative medicine including cupping. With the study of mechanism of action of cupping, it will be more convincing for the traditional medic...
متن کاملEvaluation of biochemical differences and Immunological effects of LPS and Lipid A extracted from Brucella strains
Background: The intrinsic heterogeneity determination in Brucella Lipopolysaccharide (LPS) is important for explaining its chemicalnature and biological behavior. This is significant for practical purposes, since LPS is the most relevant antigen during infectionand vaccination.Objectives: The purpose of the present study was to compare biochemical and immunological differences of LPS and lipid ...
متن کاملTHE EFFECT OF ENDURANCE TRAINING ON GENE EXPRESSION OF NERVE AND FIBROBLAST GROWTH FACTORS IN THE HIPPOCAMPUS OF RATS AFTER BRAIN STROKE
Background & Aims: Neurotrophic and growth factors are known to have positive effects on neuronal proliferation. However, findings on the effects of exercise training on these factors following brain stroke are limited. Thus, the aim of the present study was to investigate the effect of endurance training on gene expression of nerve growth factor (NGF) and fibroblast growth factor (FGF) in the ...
متن کاملA novel low molecular weight extracellular protease from a moderately halophilic bacterium Salinivibrio sp. strain MS-7: production and biochemical properties
Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and maximum production of...
متن کاملBiochemical properties and biological functions of the enzyme rhodanese in domestic animals
The enzyme rhodanese (thiosulfate: cyanide sulfurtransferase) is a ubiquitous enzyme and its activity ispresent in all living organisms. Many functions including cyanide detoxification, formation of iron-sulfurcenters and participation in energy metabolism have been attributed to this enzyme. The enzyme catalyzesthe transfer of a sulfur atom from sulfane containing compounds (such as thiosulfat...
متن کامل